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mouse anti human icam (R&D Systems)

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R&D Systems mouse anti human icam
Mouse Anti Human Icam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+icam+1/Human+ICAM-1%2FCD54+Antibody/pm41944190-237-44-47
Average 93 stars, based on 110 article reviews

mouse anti human icam - by Bioz Stars, 2026-09

93/100 stars

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Incubation:

Article Title: Development of a 3D cell-printed RVO model by advancing a retina-on-a-chip with hybrid retinal dECM bioink and an integrated 3D bioprinting system
Article Snippet: .. For target protein detection, the samples were incubated overnight at 4 °C with primary antibodies (1:100 dilution) specific to the proteins of interest, including anti-GFAP (ab7260, Abcam), anti-nestin (N5413, Sigma‒Aldrich), anti-rhodopsin (ab5417, Abcam), anti-VE-cadherin (ab33168, Abcam), anti-CD31 (ab9498, Abcam), anti-ZO-1 (33–9100, Invitrogen), anti-CD68 (MA5-13324, Invitrogen), anti-ICAM-1 (BBA3, R&D Systems), anti-E-cadherin (sc8426, Santa Cruz), anti-PDGFR (ab69506, Abcam), and anti-RPE65 (ab138226, Invitrogen). .. After incubation, the samples were washed with 1 × PBS and treated with Alexa488 and Alexa-594 secondary antibodies (1:200; Thermo Fisher Scientific) for 1 h. Nuclei were stained with DAPI (GBI Labs, USA) for 5 min at RT.

Article Title: Earlier onset of chemotherapy-induced neuropathic pain in females by ICAM-1–mediated accumulation of perivascular macrophages
Article Snippet: Cryostat sections (40 μm thick) were cut and blocked with QuickBlock Blocking Buffer for Immunol Staining (Beyotime, P0260) for 1 hour at room temperature. .. Sections were then incubated overnight at 4°C with primary antibodies against goat anti–ICAM-1 (1:100; R&D Systems, AF796), rat anti-F4/80 (1:200; Abcam, ab6640), rabbit anti-F4/80 (1:200 Abcam, ab300421), rat anti-CD31 (1:50; BD Biosciences, 550274), rabbit anti-TMEM119 (1:500; Abcam, ab209064), rabbit anti–IL-1α (1:200; Abcam, ab300500), rat anti-CCL1 (1:200; R&D Systems, MAB846), mouse anti–IL-1RI (1:50; R&D Systems, AF771), rabbit anti-CCR8 (1:50 Boster, M03518), mouse anti–glial fibrillary acidic protein (1:500; Abcam, ab4648), and mouse anti-neuron-specific nuclear protein (NeuN) (1:500; Merck, MAB377). ..

Article Title: Earlier onset of chemotherapy-induced neuropathic pain in females by ICAM-1-mediated accumulation of perivascular macrophages.
Article Snippet: Cryostat sections (40 μm thick) were cut and blocked with QuickBlock Blocking Buffer for Immunol Staining (Beyotime, P0260) for 1 hour at room temperature. .. Sections were then incubated overnight at 4°C with primary antibodies against goat anti–ICAM- 1 (1:100; R&D Systems, AF796), rat anti- F4/80 (1:200; Abcam, ab6640), rabbit anti- F4/80 (1:200 Abcam, ab300421), rat anti- CD31 (1:50; BD Biosciences, 550274), rabbit anti- TMEM119 (1:500; Abcam, ab209064), rabbit anti–IL- 1α (1:200; Abcam, ab300500), rat anti- CCL1 (1:200; R&D Systems, MAB846), mouse anti–IL- 1RI (1:50; R&D Systems, AF771), rabbit anti- CCR8 (1:50 Boster, M03518), mouse anti–glial fibrillary acidic protein (1:500; Abcam, ab4648), and mouse anti- neuron- specific nuclear protein (NeuN) (1:500; Merck, MAB377). ..

Staining:

Article Title: Microfluidic artery-on-a-chip model with unidirectional gravity-driven flow for high-throughput applications.
Article Snippet: After fixation, cultures in the OrganoPlate were stained for immunofluorescent markers, as described previously.42 In short, cells were permeabilized using a Triton X-100 solution for 15 min and blocked using a buffer containing FBS, bovine serum albumin, and Tween-20 for 45 min. Primary antibodies were incubated overnight, after which secondary antibodies were incubated for 2 hours. .. The following primary antibodies were used to stain fixed cultures: anti-VE-cadherin 1 : 500 (Abcam, ab33168), anti-ICAM-1 1 : 50 (R&D systems, BBA3), antihuman CD31 1 : 160 (Dako, M0823), fibronectin 1 : 100 (Sigma, F3648) and αSMA 1 : 500 (Sigma, A2547). .. The following secondary antibodies were used: goat anti-rabbit IgG (H + L) Alexa Fluor 555 1 : 250 (Thermo Fischer Scientific, A21428), goat anti-mouse IgG (H + L) Alexa Fluor 647 1 : 250 (Biotium, 20040).

Article Title: Interleukin-6 regulates the neutrophil response to diverse bacteria
Article Snippet: To permeabilize, a solution of 0.2% BSA (A7906, Sigma-Aldrich) and 0.1% Tween 20 (E108, Bethyl Laboratories) in PBS+ was added to each device 3 times and incubated at room temperature for 10 minutes. .. Next, a staining solution of 0.005 mg/mL Hoechst 34580 ( H21486 , Invitrogen), 5:600 anti-ICAM-1 (FITC conjugated, BBA20, R&D Systems), 1:1000 phalloidin (iFluor-555 conjugated, ab176756, Abcam), and 5:600 anti-VE-Cadherin (Alexa Fluor 647 conjugated, 561567, BD Pharmingen) was added 3 times to each device. ..

Image Search Results

(A-D) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected, and cells were fixed at 24, 48, and 72h post-infection. A) Immunofluorescence staining of ICAM-1 (green), F-actin (phalloidin; grey), and nuclei (DAPI; blue) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. B) Quantification of HMVEC-L ICAM-1 intensity analysed by 2-way ANOVA with Sidak’s multiple comparison’s test. C) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. D) Quantification of the percentage of gaps in the endothelial monolayer was analysed by 2-way ANOVA with Sidak’s multiple comparison’s test. E-H) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected, and cells were fixed at 72h post-infection. E) Immunofluorescence staining of Zombie Red (magenta) in HMVEC-L in the basal compartment of the co-culture. Images are representative of four independent experiments. F) The percentage of Zombie Red-positive cells between conditions was analysed using the Kruskal-Wallis test with Dunn’s multiple testing correction. G) Immunofluorescence staining and imaging of CellTrace Yellow-labelled platelets (yellow), VE-cadherin (magenta), and nuclei (DAPI; blue) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. H) Gaps in the cellular monolayer were outlined relative to VE-cadherin staining, and only the platelets present in gaps were quantified. I) The number of platelets (CellTrace Yellow positive particles larger than 2 mm) in gaps was quantified and analysed by the Kruskal-Wallis test with Dunn’s multiple testing correction. Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented by the large dot (colour-coded by experiment), and the data show the mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: bioRxiv

Article Title: IL-1β and TNF drive endothelial dysfunction and coagulopathy in acute COVID-19

doi: 10.64898/2026.03.21.713333

Figure Lengend Snippet: (A-D) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected, and cells were fixed at 24, 48, and 72h post-infection. A) Immunofluorescence staining of ICAM-1 (green), F-actin (phalloidin; grey), and nuclei (DAPI; blue) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. B) Quantification of HMVEC-L ICAM-1 intensity analysed by 2-way ANOVA with Sidak’s multiple comparison’s test. C) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. D) Quantification of the percentage of gaps in the endothelial monolayer was analysed by 2-way ANOVA with Sidak’s multiple comparison’s test. E-H) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected, and cells were fixed at 72h post-infection. E) Immunofluorescence staining of Zombie Red (magenta) in HMVEC-L in the basal compartment of the co-culture. Images are representative of four independent experiments. F) The percentage of Zombie Red-positive cells between conditions was analysed using the Kruskal-Wallis test with Dunn’s multiple testing correction. G) Immunofluorescence staining and imaging of CellTrace Yellow-labelled platelets (yellow), VE-cadherin (magenta), and nuclei (DAPI; blue) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. H) Gaps in the cellular monolayer were outlined relative to VE-cadherin staining, and only the platelets present in gaps were quantified. I) The number of platelets (CellTrace Yellow positive particles larger than 2 mm) in gaps was quantified and analysed by the Kruskal-Wallis test with Dunn’s multiple testing correction. Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented by the large dot (colour-coded by experiment), and the data show the mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies against mouse ICAM-1 (BioXCell; BE0020-1) and mouse CD31 (New England Biolabs; 77699S).

Techniques: Infection, Immunofluorescence, Staining, Co-Culture Assay, Imaging

(A-E) NHBE/HMVEC-L co-cultures infected with SARS-CoV-2 (MOI 1) and then treated with 100 mg/mL dexamethasone or media alone immediately post-infection. Cells were fixed at 72h post-infection. A) Immunofluorescence staining of ICAM-1 (green) in HMVEC-L in the basal compartment of the co-culture. Images are representative of 2 independent experiments. B) Quantification of HMVEC-L ICAM-1 intensity, where data shows mean + SD. C) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. D) Quantification of the percentage of gaps in the endothelial monolayer was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. E) Viral titres from the apical compartment of SARS-CoV-2-infected NHBE/HMVEC-L co-cultures, untreated or treated with 100 mg /mL dexamethasone, at 72h post-infection. n = 3 independent experiments, analysed by unpaired, two-way t -test. F) Schematic of supernatant transfer experiment. G-J) NHBE monocultures were infected with SARS-CoV-2 for 48h. The supernatant from the basal compartment was then transferred onto HMVEC-L. 100 mg /mL dexamethasone or PBS was added to the NHBE basal supernatants before they were transferred onto HMVEC-L. After 24h, HMVEC-L were fixed for immunofluorescence staining. G) Immunofluorescence staining of ICAM-1 (green) in HMVEC-L. Images are representative of 3 independent experiments. H) Quantification of HMVEC-L ICAM-1 intensity analysed by one-way ANOVA with Sidak’s multiple comparison’s test. I) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L. Images are representative of three independent experiments. J) Quantification of the percentage of gaps in the endothelial monolayer was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented by the large dot (colour-coded by experiment), and the data show the mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: bioRxiv

Article Title: IL-1β and TNF drive endothelial dysfunction and coagulopathy in acute COVID-19

doi: 10.64898/2026.03.21.713333

Figure Lengend Snippet: (A-E) NHBE/HMVEC-L co-cultures infected with SARS-CoV-2 (MOI 1) and then treated with 100 mg/mL dexamethasone or media alone immediately post-infection. Cells were fixed at 72h post-infection. A) Immunofluorescence staining of ICAM-1 (green) in HMVEC-L in the basal compartment of the co-culture. Images are representative of 2 independent experiments. B) Quantification of HMVEC-L ICAM-1 intensity, where data shows mean + SD. C) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L in the basal compartment of the co-culture. Images are representative of three independent experiments. D) Quantification of the percentage of gaps in the endothelial monolayer was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. E) Viral titres from the apical compartment of SARS-CoV-2-infected NHBE/HMVEC-L co-cultures, untreated or treated with 100 mg /mL dexamethasone, at 72h post-infection. n = 3 independent experiments, analysed by unpaired, two-way t -test. F) Schematic of supernatant transfer experiment. G-J) NHBE monocultures were infected with SARS-CoV-2 for 48h. The supernatant from the basal compartment was then transferred onto HMVEC-L. 100 mg /mL dexamethasone or PBS was added to the NHBE basal supernatants before they were transferred onto HMVEC-L. After 24h, HMVEC-L were fixed for immunofluorescence staining. G) Immunofluorescence staining of ICAM-1 (green) in HMVEC-L. Images are representative of 3 independent experiments. H) Quantification of HMVEC-L ICAM-1 intensity analysed by one-way ANOVA with Sidak’s multiple comparison’s test. I) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L. Images are representative of three independent experiments. J) Quantification of the percentage of gaps in the endothelial monolayer was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented by the large dot (colour-coded by experiment), and the data show the mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies against mouse ICAM-1 (BioXCell; BE0020-1) and mouse CD31 (New England Biolabs; 77699S).

Techniques: Infection, Immunofluorescence, Staining, Co-Culture Assay

(A-H) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected and treated with 10 mg/mL anti-TNF (Adalimumab) immediately post-infection. Cells were fixed at 72h post-infection. A) Immunofluorescence staining for ICAM-1 (green), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 3 independent experiments. B) ICAM-1 intensity between conditions was analysed by one-way ANOVA, with Sidak’s multiple comparison test. C) Immunofluorescence staining for VE-cadherin (magenta), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 3 independent experiments. D) Quantification of gaps in the endothelial monolayer under different conditions was determined by calculating the percentage of the image area covered by gaps, and analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. E) Zombie Red-stained cells (magenta) indicate cells (containing F-actin and nuclei) undergoing cell death. Images are representative of n = 3 independent experiments. F) The percentage of Zombie Red positive cells was analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. G) Immunofluorescent staining of CellTrace Yellow-labelled platelets incubated with HMVEC-L. Images are representative of n = 3 independent experiments. H) The number of platelets (CellTrace Yellow positive particles larger than 2 mm) in gaps was quantified and analysed by the Kruskal-Wallis test with Dunn’s multiple testing correction. I) NHBE monocultures and NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected, and TNF levels in the apical and basal supernatants were analysed at 24, 48, and 72h post-infection. Data show the mean ± SEM of 3 independent experiments, analysed by 2-way ANOVA. (J-M) NHBE monocultures were infected with SARS-CoV-2 for 48h. The supernatant from the basal compartment was then transferred onto HMVEC-L. Anti-TNF (10 mg/mL) or PBS was added to the NHBE basal supernatants before they were transferred onto HMVEC-L. After 24h, HMVEC-L were fixed for immunofluorescence staining. J) Immunofluorescence staining of ICAM-1 (green) in HMVEC-L. Images are representative of 3 independent experiments. K) Quantification of HMVEC-L ICAM-1 intensity was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. L) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L. Images are representative of three independent experiments. M) Quantification of the percentage of gaps in the endothelial monolayer was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented with the large dot (colour-coded per experiment), and the data shows mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: bioRxiv

Article Title: IL-1β and TNF drive endothelial dysfunction and coagulopathy in acute COVID-19

doi: 10.64898/2026.03.21.713333

Figure Lengend Snippet: (A-H) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected and treated with 10 mg/mL anti-TNF (Adalimumab) immediately post-infection. Cells were fixed at 72h post-infection. A) Immunofluorescence staining for ICAM-1 (green), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 3 independent experiments. B) ICAM-1 intensity between conditions was analysed by one-way ANOVA, with Sidak’s multiple comparison test. C) Immunofluorescence staining for VE-cadherin (magenta), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 3 independent experiments. D) Quantification of gaps in the endothelial monolayer under different conditions was determined by calculating the percentage of the image area covered by gaps, and analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. E) Zombie Red-stained cells (magenta) indicate cells (containing F-actin and nuclei) undergoing cell death. Images are representative of n = 3 independent experiments. F) The percentage of Zombie Red positive cells was analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. G) Immunofluorescent staining of CellTrace Yellow-labelled platelets incubated with HMVEC-L. Images are representative of n = 3 independent experiments. H) The number of platelets (CellTrace Yellow positive particles larger than 2 mm) in gaps was quantified and analysed by the Kruskal-Wallis test with Dunn’s multiple testing correction. I) NHBE monocultures and NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected, and TNF levels in the apical and basal supernatants were analysed at 24, 48, and 72h post-infection. Data show the mean ± SEM of 3 independent experiments, analysed by 2-way ANOVA. (J-M) NHBE monocultures were infected with SARS-CoV-2 for 48h. The supernatant from the basal compartment was then transferred onto HMVEC-L. Anti-TNF (10 mg/mL) or PBS was added to the NHBE basal supernatants before they were transferred onto HMVEC-L. After 24h, HMVEC-L were fixed for immunofluorescence staining. J) Immunofluorescence staining of ICAM-1 (green) in HMVEC-L. Images are representative of 3 independent experiments. K) Quantification of HMVEC-L ICAM-1 intensity was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. L) Immunofluorescence staining of VE-cadherin (magenta) in HMVEC-L. Images are representative of three independent experiments. M) Quantification of the percentage of gaps in the endothelial monolayer was analysed by one-way ANOVA with Sidak’s multiple comparison’s test. Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented with the large dot (colour-coded per experiment), and the data shows mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies against mouse ICAM-1 (BioXCell; BE0020-1) and mouse CD31 (New England Biolabs; 77699S).

Techniques: Infection, Immunofluorescence, Staining, Comparison, Incubation

(A-F) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected and treated with 10 mg/mL Anakinra immediately post-infection. Cells were fixed at 72h post-infection. A) Immunofluorescence staining for ICAM-1 (green), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 5 independent experiments. B) ICAM-1 intensity between conditions was analysed by one-way ANOVA, with Sidak’s multiple comparison test. C) Immunofluorescence staining for VE-cadherin (magenta), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 3 independent experiments. D) Quantification of gaps in the endothelial monolayer under different conditions was determined by calculating the percentage of the image area covered by gaps, and analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. E) Zombie Red-stained cells (magenta) indicate cells (containing F-actin and nuclei) undergoing cell death. Images are representative of n = 3 independent experiments. F) The percentage of Zombie Red positive cells was analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. G) IL-1β levels in the apical supernatant of NHBE monocultures infected with SARS-CoV-2 (MOI 1) or mock-infected, at 24, 48, and 72h post-infection (n = 3 independent experiments). H) TNF levels in the apical (left panel) and basal (right panel) supernatant of SARS-CoV-2-infected NHBE/HMVEC-L co-cultures, untreated or treated with Anakinra, at 24, 48, and 72h post-infection (n = 2 independent experiments). Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented with the large dot (colour-coded per experiment), and the data shows mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: bioRxiv

Article Title: IL-1β and TNF drive endothelial dysfunction and coagulopathy in acute COVID-19

doi: 10.64898/2026.03.21.713333

Figure Lengend Snippet: (A-F) NHBE/HMVEC-L co-cultures were infected with SARS-CoV-2 (MOI 1) or mock-infected and treated with 10 mg/mL Anakinra immediately post-infection. Cells were fixed at 72h post-infection. A) Immunofluorescence staining for ICAM-1 (green), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 5 independent experiments. B) ICAM-1 intensity between conditions was analysed by one-way ANOVA, with Sidak’s multiple comparison test. C) Immunofluorescence staining for VE-cadherin (magenta), F-actin (Phalloidin; grey), and nuclei (DAPI; blue). Images are representative of n = 3 independent experiments. D) Quantification of gaps in the endothelial monolayer under different conditions was determined by calculating the percentage of the image area covered by gaps, and analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. E) Zombie Red-stained cells (magenta) indicate cells (containing F-actin and nuclei) undergoing cell death. Images are representative of n = 3 independent experiments. F) The percentage of Zombie Red positive cells was analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. G) IL-1β levels in the apical supernatant of NHBE monocultures infected with SARS-CoV-2 (MOI 1) or mock-infected, at 24, 48, and 72h post-infection (n = 3 independent experiments). H) TNF levels in the apical (left panel) and basal (right panel) supernatant of SARS-CoV-2-infected NHBE/HMVEC-L co-cultures, untreated or treated with Anakinra, at 24, 48, and 72h post-infection (n = 2 independent experiments). Scale bar for all images = 50 µm. 5 ROIs per experiment were quantified (small dots) and are colour-coded per experiment. The average of the 5 ROIs is represented with the large dot (colour-coded per experiment), and the data shows mean ± SEM. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies against mouse ICAM-1 (BioXCell; BE0020-1) and mouse CD31 (New England Biolabs; 77699S).

Techniques: Infection, Immunofluorescence, Staining, Comparison

A) Representative immunohistochemistry (IHC) images of lungs from SARS-CoV-2-infected (10 4 TCID50) or mock-infected wild-type (WT), Tnf -/- , Il1b -/- , and Tnf -/- IL1b -/- mice, harvested at 3 days post-infection (dpi). Tissues were stained with CD31 (magenta), ICAM-1 (green), and DAPI (blue). Scale bar = 50 µm. Images are representative of 5 images per mouse, 3 mice per group. B) Quantification of ICAM-1 intensity in areas of CD31 staining, analysed by 2-way ANOVA with Sidak’s multiple testing correction. 5 ROIs per mouse were quantified (small dots) and are colour-coded per mouse. The average of the 5 ROIs is represented with the large dot (colour-coded per mouse), and the data shows mean ± SEM. C-F) Aged K18-hACE c57BL/6□J mice infected with SARS-CoV-2 (10 3 PFU) and treated with an isotype control or anti-IL-1β antibody at 1h or 3 days post-infection. Lungs were harvested at day 6 post-infection. C) Lung tissues were stained with CD31 (magenta), ICAM-1 (green), and DAPI (blue). Scale bar = 100 µm. Images are representative of 5 images per mouse, 4-6 mice per group. D) Quantification of ICAM-1 intensity in areas of CD31 staining, analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. 5 ROIs per mouse were quantified (small dots) and are colour-coded per mouse. The average of the 5 ROIs is represented by the large dot (colour-coded per mouse), and the data show the mean ± SEM. E) Lung tissues were stained for fibrinogen. Scale bar = 50 µm. Images are representative of 4-6 mice per group. F) Quantification of fibrinogen staining intensity analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: bioRxiv

Article Title: IL-1β and TNF drive endothelial dysfunction and coagulopathy in acute COVID-19

doi: 10.64898/2026.03.21.713333

Figure Lengend Snippet: A) Representative immunohistochemistry (IHC) images of lungs from SARS-CoV-2-infected (10 4 TCID50) or mock-infected wild-type (WT), Tnf -/- , Il1b -/- , and Tnf -/- IL1b -/- mice, harvested at 3 days post-infection (dpi). Tissues were stained with CD31 (magenta), ICAM-1 (green), and DAPI (blue). Scale bar = 50 µm. Images are representative of 5 images per mouse, 3 mice per group. B) Quantification of ICAM-1 intensity in areas of CD31 staining, analysed by 2-way ANOVA with Sidak’s multiple testing correction. 5 ROIs per mouse were quantified (small dots) and are colour-coded per mouse. The average of the 5 ROIs is represented with the large dot (colour-coded per mouse), and the data shows mean ± SEM. C-F) Aged K18-hACE c57BL/6□J mice infected with SARS-CoV-2 (10 3 PFU) and treated with an isotype control or anti-IL-1β antibody at 1h or 3 days post-infection. Lungs were harvested at day 6 post-infection. C) Lung tissues were stained with CD31 (magenta), ICAM-1 (green), and DAPI (blue). Scale bar = 100 µm. Images are representative of 5 images per mouse, 4-6 mice per group. D) Quantification of ICAM-1 intensity in areas of CD31 staining, analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. 5 ROIs per mouse were quantified (small dots) and are colour-coded per mouse. The average of the 5 ROIs is represented by the large dot (colour-coded per mouse), and the data show the mean ± SEM. E) Lung tissues were stained for fibrinogen. Scale bar = 50 µm. Images are representative of 4-6 mice per group. F) Quantification of fibrinogen staining intensity analysed by one-way ANOVA, with Sidak’s multiple comparison’s test. Asterisks indicate statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibodies against mouse ICAM-1 (BioXCell; BE0020-1) and mouse CD31 (New England Biolabs; 77699S).

Techniques: Immunohistochemistry, Infection, Staining, Control

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